Identifying a peak is different from measuring one.
Classic chromatography tells you how much of a substance is present, provided you already know what you are looking for. LC-MS and GC-MS add a dimension: molecular identity. For unknown compounds, complex matrices, or questions at the cutting edge of detection, this coupling is indispensable.
LC-MS and GC-MS are therefore relevant in every sector we serve.
Mass spectrometry adds certainty where UV detection leaves doubt.
LC-MS and GC-MS combine separation with mass spectrometric identification for questions that go beyond simple quantification.
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Choosing between LC-MS and GC-MS
The physicochemical properties of the target component (volatility, polarity, thermal stability) determine which technique provides the correct separation and ionization.
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Detection at very low concentrations
Mass spectrometry enables detection at ppb and ppt levels, which is essential for impurity analysis and trace compounds that other detectors cannot identify.
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Identification of unknown components
Using fragmentation patterns and mass spectra, we can elucidate the structure of unknown compounds, rather than just confirming their presence.
Expertise
Reliability
Flexibility
Partnership
From unknown peak to confirmed identity.
Our LC-MS and GC-MS approach combines chromatographic separation with thorough mass spectrometric interpretation.
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Optimizing mass spectrometry
For the LC-MS method, we optimize ionization using ESI or APCI, depending on the properties of the target substances. We then determine the optimal precursor and product ions, MRM transitions, collision energies, and other MS settings. This allows us to achieve the highest possible sensitivity and selectivity. For GC-MS(MS), we optimize ionization and MS settings based on the properties of the target substances. Depending on the application, we select suitable characteristic ions for full-scan, SIM, or MRM analysis and optimize parameters such as precursor and product ions and collision energies. This allows us to achieve the highest possible sensitivity, selectivity, and reliable identification.
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Optimizing chromatography
Next, we optimize the chromatographic separation, including the column, mobile phase, gradient, and analysis time. For GC, we optimize the column type, injection technique, temperature program, carrier gas settings, and analysis time. The goal is a reliable separation of the target substances, interferences, and any isomers. In doing so, we seek the right balance between resolution, sensitivity, and efficiency.
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Matrix introduction and sample preparation
Once the instrumental method is optimized, we introduce the actual sample matrix. We investigate potential matrix effects and optimize sample preparation, extraction, and clean-up. Where necessary, we apply internal standards to correct for variations in recovery and ionization.
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Method qualification and validation
Depending on the intended use, the developed LC-MS(/MS) or GC-MS(/MS) method can be qualified as scientifically sound or fully validated according to ICH Q2(R2). For a scientifically sound method, we assess selectivity, accuracy, and detection limits based on the principles of ICH Q2(R2). For pharmaceutical applications, a full validation can be performed according to a pre-approved protocol with established acceptance criteria, followed by a comprehensive validation report.
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Sectors where method development is essential.
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Insights from our laboratory
Brightlabs at CPHI Milan
Meet Brightlabs at CPHI Milan. Brightlabs will be attending CPHI Milan from 6 to 8 October 2026. We would be delighted to discuss your analytical challenges and explore how our expertise in chemical and microbiological analyses can support your product development, quality control and market launch.
The complete overview of what Brightlabs does.
Download our brochure for a complete overview of our analytical capabilities, services, and scientific approach.
